| Unlockable: | How to Unlock: |
| April's adventure mode | As Donatello, successfully complete the Ghetto level. |
| April's Apartment stage | As Donatello, successfully complete the April's Apartment 2 level. |
| April's melee mode | As Casey, successfully complete adventure mode. |
| Autumn stage | As Michaelangelo, successfully complete The Dojo level. |
| Casey Jones' adventure mode | As Raphael, successfully complete the Subway level. |
| Casey Jones' jacket costume | As April, successfully complete her adventure mode. |
| Casey Jones' melee mode | As Casey Jones, successfully complete adventure mode. |
| Casey Jones' red shorts costume | As Casey Jones, successfully complete level the Ghetto level. |
| Dojo Complete stage | As Casey Jones, successfully complete the Turtle's Lair level. |
| Foot Soldier's adventure mode | As Raphael, successfully complete his adventure mode. |
| HUN 2's melee mode | As HUN, successfully complete his adventure mode. |
| HUN's adventure mode | As Michaelangelo, successfully complete his adventure mode. |
| Monster's melee mode | As Hun, successfully complete the Sewer Canal level. |
| Sewer Falls stage | As Raphael, successfully complete the Sewer Falls level. |
| Sewers stage | As Leonardo, successfully complete the Sewers level. |
| Shredder's adventure mode | As Donatello, successfully complete his adventure mode. |
| Shredder's gold costume | As Shredder, successfully complete his adventure mode. |
| Shredder's Palace Exterior stage | As Leonardo, successfully complete The Lab 12 level. |
| Splinter's adventure mode | As Leonardo, successfully complete his adventure mode. |
| Subway stage | As Leonardo, successfully complete the Sparring With Splinter level. |
| Tech Foot's melee mode | As Foot Soldier, successfully complete adventure mode. |
| Waterfall stage | As April, successfully complete the Turtle's Lair level. |